UV/Vis Photometric Performance: Wavelength Range. Find regional support. spr SpectraMax iD3 SpectraTest ABS2 v1. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Depot. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Here, we describe the setup of a bacterial growth assay using SoftMax ® Pro 7. 1. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Find regional support. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. SoftMax Pro Software can collect data from one or more microplates orSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. FlexStation® 3 Multi-Mode Microplate Reader. The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 µg/mL of G418. com. What is fluorescence? Fluorescence is the property of some atoms and molecules to absorb light at a particular wavelength (the excitation: Ex) followed by a short-lived emission (Em) of light at a longer wavelength (Figure 2). 1. 1. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 313 reference gain check fail. Detection cartridge modularity allows for easy addition of new read modes within minutes without. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). Preconfigured protocols in SoftMax Pro Software expedite data. STAKMAX. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Prepare your i3/i3x instrument for shipment. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. HEALTH AND SAFETY CONSIDERAIONS SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. com A. Check software compatibility with Windows 10 and locate your software installer. Compatible with SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, FlexStation 3 readers 0200-6191: Gray replacement validation plate case Compatible with. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. Measuring cell health on the SpectraMax iD3 reader with cell viability assays. SpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices) Methods Cell preparation and analysis. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Also refer to this Multimode Reader Comparison Chart. With optimized reagents, option to operate the readers. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Assay results are similar on the SpectraMax M5 or other Molecular Devices readers with fluorescence detection mode. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi-ModeMicroplateReader SpectraMax® SpectraMaxLMicroplateReader For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way? SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraTest ABS1 Validation Plate is a comprehensive optical validation package that is com-SpectraMax® Microplate Reader Suite of Products Knowledge Base. 7. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. SpectraMax M-series Microplate readers. 1700 +1 800. (Molecular Devices Cat. While they play an important role in generating data from a wide range of. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Microplate Readers include the M3, M4,. Molecular Devices SpectraMax M5 Plate Reader Repair. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Check: In the data table create two new columns and add the following formulas: !Pathchecklm900. Step 4: Incubate at room temperature for 1 hour, protected from light. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. It is possible to search for the Overlord device driver for the instrument of your choice. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Fluorescence Microplate Reader Comparison Chart. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. We would like to show you a description here but the site won’t allow us. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. View the Molecular Devices website. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Wait 20 seconds. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Sensitive fluorescent quantitation of DNA with the Quant-iT. 635. SpectraMax M5 is ideal for laboratories requiring maximum flexibility in the detection of a. +1 800-635-5577. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. The SpectraMax 190 reader can be integrated with Molecular . CHO M1WT3 antagonist IC50 curves. Histamine H1 is an endogenous receptor in HeLa cells. Description 1. 4. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. It has patented optimization, IQ/OQ/PM services,. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. All associated StakMax® stackers. The CyQUANT Cell Proliferation Assay is a quick, sensitive fluorescence method for quantitating cell numbers or cellular DNA content. The smaller PMT is. The StakMax system is an easy-to-set-up, powerful, and reliable stacking solution for. Z-height and microplate optimization increases assay dynamic range and sensitivity. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. We would like to show you a description here but the site won’t allow us. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. STAKMAX. Bottom-reading is usually recommended for cell-based assay with adherent cells. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multi. On-site Repair Service available nationwide for Molecular Devices SpectraMax M5 Plate Reader's. keeping with best practices, reader performance should. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 1. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 1. S. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. To support these assays, we provide application notes as well as. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Supplier: Molecular Devices. 005) Photometric PrecisionScanLater Western Blot Detection Cartridge. To support these assays, we provide application notes as well as ready-to-run protocols in our SoftMax Pro Software. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. IMAP technology is based on. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Download PDF. cuvette port for assay development • Dual monochromator tunability • Automated absorbance pathlength correction The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dualmonochromator microplate reader. We would like to show you a description here but the site won’t allow us. View the Molecular Devices website. Plate chamber design of the SpectraMax category 1 microplate readers. Table of Contents. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. . Various data transformation steps in the software are discussed, such as normalizing the GFP. If values are present, then. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Unplug the power cable from the back of the instrument. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. +44-118-944-8000. Our. Products : SpectraMax M2/M2e reader, FlexStation II (Obsolete), FlexStation 3 reader, SpectraMax M5/M5e reader. After running the LM1 Plate protocol, not all values in the values in Results sections are displayed; what should I do? This is a SoftMax Pro software problem rather than anything specific to the LM1 plate, protocol or reader. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Call 516-939-0888 to check current. on the SpectraMax Multi-Mode Microp late Readers by issuing a single command in SoftMax ® Pro Software, Molecular Devices leading microplate data acquisition and analysis software platform. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. Using a cell-based standard curve and a five-minute incubation, we observed a limit of. Figure 3. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Cavicide (Warehouse, Cat # 79300360) 6. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraMax ® Paradigm. 1. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Multimode Readers Comparison Chart. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, SpectraMax Plus 384 reader Why is the instrument keypad inactive? When connected to SoftMax Pro software during a run, the SpectraMax Series plate reader keypad will be inactivated for. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. It offers five preferred detection modes, wide range of applications, low volume. 100 readings per well gives optimal Z’ values for this assay. 3. The drawing shows a standard microplate of 15 mm height covered with a lid. 2. 6. This document is provided to customers who have purchased Molecular Devices, Inc. SpectraMax M5 microplate reader. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. View the Molecular Devices websiteFind answers to your questions about Molecular Devices products and software in our global knowledge base, or contact our technical support team for assistance. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Failed to load PDF file. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator 6. The. 0200-7027. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. View the Molecular Devices websiteすべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. edu) to be added as a trained user in iLab. 1) EarlyTox NucView 488 Assay (6. Cavicide (Warehouse, Cat # 79300360) 6. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. Sale. If depot service is the best option for you we will assist you with logistics to help you keep. ATP standard curves read on SpectraMax microplate readers. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Figure 2. SpectraMax 250 User. Lower end of DNA standard curve. SoftMax Pro Software can collect data from one or more microplates orSpectraMax ABS Plus SpectraTest ABS2 v1. 2. Monday to Friday 8am - 5pm GMT. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. 1 ng/mL to 50 pg/mL (r2 = 1. Optimal instrument settings are the same for all three readers. Assay Performance with Various Instrument. The SpectraMax M2 and M2e are versatile and reliable. minutes and begin reading microplates with seven mouse clicks. 3. View the Molecular Devices websiteMolecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except asIMAP® Technology from Molecular Devices enables rapid, non-radioactive assay of a wide array of kinases and is suited to both assay development and high-throughput screening. SpectraMax M5 reader SpectraMax M5eThe integration time suggested is 5-10 sec. 747. Microplate Reader Shelf. -43. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Spectranet customer portal. 6. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Future ready The SpectraMax i3 x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Select Update Driver. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. Published Dec 16, 2020 | Updated Dec 16, 2020. #R7159) APPLICATION NOTE 如何使用SpectraMax M5 多功能微孔. SpectraMax® iD5. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Fluorescence Microplate Reader Comparison Chart. 00 USD. Add to Cart. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre-configured protocol provided for customers using Molecular Devices’ SoftMax. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. M2. 2. #6590-0137) • SpectraMax i3x Multi-Mode Microplate Reader • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Molecular Devices. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next- BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. Instrument settings used with the SpectraMax M5 Microplate Reader. 2. book Page 1 Wednesday, July 7, 2010 2:09 PM. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. If no sound is heard during initialization, contact technical support. 00 shippingdetection in the Molecular Devices SpectraMax M5 device. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Table 2. Choose cables that meet the requirement described in this appendix. Phone: +1. : 915 515 403 • Fax: 914 334 545 • e-mail: [email protected] 1 1 10 10 100 100 1000 1000 10000 0. The system provides automation for up to 50The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. Fully optimized assays for SpectraMax iD3 reader save time on assay development. Results Luciferase emission spectrum. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Order Now. Anne T. to Molecular Devices for recertification at our ISO 17025. . Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. #6590-0118) Filter 665/10 (Molecular Devices cat. Plot of the lower end of the DNA standard curve, ranging from 4. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Cells were treated with increasing concentrations of the antagonist atropine and assayed for response to addition of an EC80 concentration of the agonist carbachol. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. Description 1. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. 5. ] Example of document to evaluate if the reader . OPTICSSpectraMax Glo and DuoLuc. Multimode Readers Comparison Chart. Fluorescence Microplate Reader Comparison Chart. Unit successfully connected to software, and passes self tests. 72 MB. Molecular Devices is ISO 17025 accredited† by American Association of Laboratory Accreditation (A2LA), which requires rigorous outside auditing on a regular basis so that you can be assured of the accuracy of your certificate with respect to NIST and NMI traceable standards. SpectraMax Glo Steady-Luc Reporter Assay Kit Luciferase measurement for your microplate reader assay KEY FEATURES • Glow-type luminescence provides an extended signal time. 303 unable to cal dark current. We would like to show you a description here but the site won’t allow us. Select the Driver tab. Table of Contents. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. 1. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. Description 1. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orMolecular Devices. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. Turn on the microplate reader. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Both cell density and GFP signals were recorded over a time course using the Workflow Editor acquisition function. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Figure 2. Feb 22, 2023By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. -54. ® reader to the computer. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The M5 has two PMTs. Molecular Devices, LLC. 3 mm. 72 MB. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. on the SpectraMax Multi-Mode Microp late Readers by issuing a single command in SoftMax ® Pro Software, Molecular Devices leading microplate data acquisition and analysis software platform. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. AquaMax® 2000. White plates reflect the light, enabling even low levels of Luminescence to be detected. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175) Sunnyvale, CA - May 17, 2011 - Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax® M5e Multi-Mode Microplate Reader for use in NASA's International Space Station; the M5e Reader will be the first microplate reader to be used in space. SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. i3. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Note: In this user guide, all references to SpectraMax Multi-Mode. 4. SpectraMax® Aquamax. 190-1000 nm, tunable in 1 nm increments. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86We would like to show you a description here but the site won’t allow us. 2. Lower limits of detection for ATP determined using the ATPliteThe Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. ®The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent). Table 1. 1. #14-276) 。FAM标记多肽底物 (Molecular Devices Cat. The following document is intended to demonstrate setup of this instrument. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence, luminescence, TRF and HTRF. This reader's dual monochromators for. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeMD Spectramax iD5 Plate Reader. 2.